Postdoctoral Research Associate in Designed Protein Vaccines

Working at Durham University

A globally outstanding centre of teaching and research excellence, a warm and friendly place to work, a unique and historic setting - Durham is a university like no other.

As one of the UK's leading universities, Durham is an incredible place to define your career. The University is located within a beautiful historic city, home to a UNESCO World Heritage Site, and surrounded by stunning countryside. Our talented scholars and researchers from around the world are tackling global issues and making a difference to people's lives.

We believe that inspiring our people to do outstanding things at Durham enables Durham people to do outstanding things in the world. Being a part of Durham is about more than just the success of the University, it's also about contributing to the success of the city, county and community.

Our University Strategy is built on three pillars of research, education and wider student experience, but also on our keen sense of community and of inspiring others to achieve their potential.

Our Purpose and Values

We want our University to be a place where people can be free to be themselves, no matter what their identity or background. Together, we celebrate difference, value one another and are each responsible for creating an inclusive community that is respectful and fair for all.

Find out more about the benefits of working at the University and what it is like to live and work in the Durham area on our Why Join Us? - Information Page

The Role and Department

The Department of Biosciences is composed of 62 academic staff and research fellows; around 50 research and technical support staff; over 90 Research Postgraduate students and over 500 Single Honours undergraduate students, and also teaches students from the Natural Sciences programme. The Department is committed to an ethos of research-led teaching at both BSc and MBiol levels, and was ranked 5th in the UK for Biological Sciences Departments in the Complete University Guide 2024.

Our basic science underpins applications across each-and-every sector of the bio-economy, including Biotechnology, Ecology and Agritechnology. Research programmes are grouped in the following areas: Molecular Plant Sciences; Biomolecular Interactions; Ecology, Evolution and Environment; and Animal Cells and Systems. These groups organise focused seminar series, Research Away Days, manage joint research facilities, oversee mentoring, internal peer review and serve to nucleate larger research projects. Along with our excellent research facilities such as bioimaging, proteomics, metabolomics and genomics we offer a high quality and supportive environment for our research staff.

The Dept. of Biosciences partners with other areas of research strength in Durham University including Computational Sciences and Chemistry, and encourages multidisciplinary research for Health and Wellbeing. Durham University was listed in the Times Higher World Top 50 Life Science Challengers (July 2017).


The Role

Join us as a Post-Doctoral Research Associate at the newly established Centre for Programmable Biological Matter.

At the Centre for Programmable Biological Matter, we are interested in understanding, designing and building natural and artificial biological nanomachines. We look at the amazing variety of such machines in nature, including in our own cells ask - can we design artificial versions of such machines to carry out processes not available or even not possible in nature? The ability to design functional, dynamic proteins will revolutionise medicine. It will mean new capabilities in vaccines and drug including nanoscale medicine that can be programmed and can move. We have established the Centre of Programmable Biological Matter as to spearhead research in this area.

To find out more about our research, head to our website: https://heddlelab.org/

Project description - 'Developing Artificial Protein Cages for Medicine'.

Inthis project, you join our multidisciplinary team and will lead a project to design and produce an artificial, dynamic protein cage-based vaccine able to stimulate humoral responses, gain cell entry, and open within the cell to release cargoes that further stimulate immune response. This will primarily involve two work-packages, a) Protein-Cage Design and Engineering, and b) Protein Construction, validation and testing. The work is funded by the prestigious Academy of Medical Sciences.

We have already created and characterized a stable artificial protein cage, TRAP-cage, resembling a small virus capsid with controllable dynamic properties. This means the cage can be programmed to open on demand. We also showed that folded proteins can be displayed in multiple copy number, on the exterior of the protein while folded proteins and peptides can be placed into the lumen of the cage - foundational results for vaccines and drug delivery respectively. These cages are highly stable but the conditions under which they open can be programmed, depending on the design. Programmed opening conditions shown to date include in the cytoplasm of the cell and we have shown that cages carrying cargoes can enter cells and open to free cargo. We have also demonstrated cages responsive to low pH. We have identified Lassa Fever as a suitable vaccine target. Its reach is expanding due to climate change and there is currently no human vaccine.

The location for this role will be 100% on-site in our newly refurbished labs with state-of-the art equipment, located in the Department of Chemistry, Durham University.

What will you do?

Innovate and Build: Use protein design and protein biochemistry to create hybrid structures with unprecedented structural and functional capabilities.

Utilise Advanced Tools: Harness the power of state-of-the-art lab-based biochemistry with latest in protein design software, including Deep Learning methods.

Experiment and Discover: Apply molecular biology and biochemistry techniques to craft and evaluate advanced systems, pushing the boundaries of what's possible.

Explore and Confirm: Master the art of cryo-electron microscopy to isolate, understand, and validate the structures of the systems you develop.

Collaborate and Advance: Take the most promising dynamic nanostructures to the next level in cellular studies, working alongside experienced collaborators.

Key responsibilities:

• To understand and convey material of a specialist or highly technical nature to the team or group of people through presentations and discussions that leads to the presentation of research papers in conferences and publications.
• To prepare and deliver presentations on research outputs/activities to audiences which may include: research sponsors, academic and non-academic audiences.
• To publish high quality outputs, including papers for submission to peer reviewed journals and papers for presentation at conferences and workshops under the direction of the Principal Investigator or Grant-holder.
• To assist with the development of research objectives and proposals.
• To conduct individual and collaborative research projects under the direction of the Principal Investigator or Grant-holder.
• To work with the Principal Investigator or Grant-holder and other colleagues in the research group, as appropriate, to identify areas for research, develop new research methods and extend the research portfolio.
• To deal with problems that may affect the achievement of research objectives and deadlines by discussing with the Principal Investigator or Grant-holder and offering creative or innovative solutions.
• To liaise with research colleagues and make internal and external contacts to develop knowledge and understanding to form relationships for future research collaboration.
• To plan and manage own research activity, research resources in collaboration with others and contribute to the planning of research projects.
• To deliver training in research techniques/approaches to peers, visitors and students as appropriate.
• To be involved in student supervision, as appropriate, and assist with the assessment of the knowledge of students.
• To contribute to fostering a collegial and respectful working environment which is inclusive and welcoming and where everyone is treated fairly with dignity and respect.
• To engage in wider citizenship to support the department and wider discipline.
• To engage in continuing professional development by participation in the undergraduate or postgraduate teaching programmes or by membership of departmental committees, etc. and by attending relevant training and development courses.

This post is fixed term for 24 months with a flexible expected start date prior to April 1st 2025.

The post-holder is employed to work on research/a research project which will be led by another colleague. Whilst this means that the post-holder will not be carrying out independent research in his/her own right, the expectation is that they will contribute to the advancement of the project, through the development of their own research ideas/adaptation and development of research protocols.

Working at Durham

A competitive salary is only one part of the many fantastic benefits you will receive if you join the University: you will also receive access to the following fantastic benefits:

• 30 Days annual leave per year in addition to 8 public holidays and 4 customary days per year - a total of 42 days per year.
• The University closes between Christmas and New Year - please include or delete if not applicable.
• We offer a generous pension scheme, As a new member of staff you will be automatically enrolled into the University Superannuation Scheme (USS).
• No matter how you travel to work, we have you covered. We have parking across campus, a cycle to work scheme which helps you to buy a bike and discount with local bus and train companies.
• There is a genuine commitment to developing our colleagues professionally and personally. There is a comprehensive range of development courses, apprenticeships and access to qualifications and routes to develop your career in the University. All staff have dedicated annual time to concentrate on their personal development opportunities.
• Lots of support for health and wellbeing including discounted membership for our state of the art sport and gym facilities and access to a 24-7 Employee Assistance Programme.
• On site nursery is available plus access to holiday camps for children aged 5-16.
• Family friendly policies, including maternity and adoption leave, which are among the most generous in the higher education sector (and likely above and beyond many employers).
• The opportunity to take part in staff volunteering activities to make a difference in the local community
• Discounts are available via our benefits portal including; money off at supermarkets, high street retailers, IT products such as Apple, the cinema and days out at various attractions.
• A salary sacrifice scheme is also available to help you take advantage of tax savings on benefits.
• If you are moving to Durham, you may be eligible for help with removal costs and we have a dedicated team who can help you with the practicalities such as house hunting and schools. If you need a visa, we cover most visa costs and offer an interest free loan scheme to pay for dependant visas.

Discover more about our total rewards and benefits package here.

Durham University is committed to equality, diversity and inclusion

Equality, diversity, and inclusion (EDI) are a key component of the University's Strategy and a central part of everything we do. We also live by our Purpose and Values and our Staff Code of Conduct. At Durham we actively work towards providing an environment where our staff and students can study, work and live in a community which is supportive and inclusive. It's important to us that all colleagues undertake activities that are aligned to both our values and commitment to EDI.

We welcome and encourage applications from those who are currently under-represented in our work force, including people with disabilities and from racially minoritised ethnic groups.

If you have taken a career break or periods of leave that may have impacted on the volume and recency of your research outputs and other activities, such as maternity, adoption or parental leave, you may wish to disclose this in your application. The selection committee will take this into account when evaluating your application.

The University has been awarded the Disability Confident Employer status. If you are a candidate with a disability, we are committed to ensuring fair treatment throughout the recruitment process. We will make adjustments to support the interview process wherever it is reasonable to do so and, where successful, reasonable adjustments will be made to support people within their role.

Contact Information

Department contact for academic-related enquiries

Professor Jonathan Heddle - jonathan.g.heddle@durham.ac.uk

Contact information for technical difficulties when submitting your application

If you encounter technical difficulties when using the online application form, we prefer you send enquiries by email. Please send your name along with abrief description of the problem you're experiencing toe.recruitment@durham.ac.uk

Alternatively, you may call 0191 334 6801 from the UK, or +44 191 334 6801 from outside the UK. This number operates during the hours of 09.00 and 17.00 Monday to Friday, UK time. We will normally respond within one working day (Monday to Friday, excluding UK public holidays).

University contact for general queries about the recruitment process

e.recruitment@durham.ac.uk

How to Apply

To progress to the assessment stage, candidates must evidence each of the essential criteria required for the role in the person specification below. It will be at the discretion of the recruiting panel as to whether they will also consider any desirable criteria, but we would urge candidates to provide evidence for all criteria.

While some criteria will be considered at the shortlisting stage, other criteria may be considered later in the assessment process, such as questions at interview.

Submitting your application

We prefer to receive applications online. We will update you about your application at various points throughout the selection process, via automated emails from our e-recruitment system. Please check your spam/junk folder periodically to make sure you have not missed any of our updates.

What to Submit

All applicants are asked to submit:

• a CV and cover letter which details your experience, skills, and achievements in meeting (or the potential to meet) the criteria set out below.

Next Steps

Short-listed candidates will be invited to the University, either virtually or in-person and will have the opportunity to meet key members of the Centre. The assessment for the post will normally include a presentation to staff and students from the Centre.

Please note that in submitting your application Durham University will be processing your data. We would ask you to consider the relevant University Privacy Statement Job Applicants/Potential Job Applicants - Durham University which provides information on the collation, storing and use of data.

When appointing to this role the University must ensure that it meets any applicable immigration requirements, including salary thresholds which are applicable to some visas.
Person Specification

Essential Criteria:

Qualifications

A good first degree in Biological sciences, including but not limited to protein chemistry, biochemistry, biophysics and structural biology, computational biology, computer sciences, natural sciences, mathematics.

A PhD (or be close to submission) in structural biology, protein engineering, physical virology or a related subject.

Experience

A proven track record in protein biochemistry. Work on protein cages, viral capsids or VLPS would be an advantage but not obligatory

Experience in molecular biology and protein expression and production

Experience in at least one protein structure related method e.g. cryo-EM, SAXS, NMR, protein crystallography etc.

Expertise in methods of protein -protein conjugation would be useful but nor obligatory

Skills

Strong publication record in peer-reviewed journals, commensurate with stage of career.

Demonstrable ability to work cooperatively as part of a team, including participating in research meetings.

Ability to work independently on own initiative and to strict deadlines.

Excellent interpersonal and communication skills.

Desirable Criteria

Experience

11. Demonstrable ability to develop research proposals and designs in collaboration with other academics.

12. Experience of overseeing students with respect to the development of their practical/research skills e.g. acting as a demonstrator; supervising student projects/practicals.

Skills

13. Demonstrable ability to plan and manage independent research.