Research Assistant or Associate in Therapeutic Expression
Publicerad 2025-02-21
A Research Associate is sought to work on the expression of therapeutic proteins and vaccines in the yeast Pichia pastoris with particular emphasis on virus-like particles and monoclonal antibodies. The post is part of the UKRI-funded Engineering Biology Mission Hub GlycoCell.
The project will involve strain engineering, small-scale production and purification of proteins in bioreactors, and the characterisation of their quality (assembly state, glycosylation, etc).
The following essential knowledge, skills & experience are sought:
PhD in life sciences, bioengineering, (bio)chemical engineering, biotechnology or a closely related discipline, or equivalent research, industrial or commercial experience*Practical experience in a broad range of techniques including Pichia pastoris engineering (genetic construct design, transformation, colony selection)Practical experience in protein characterisation assays, particularly in the determination of virus-like particle assembly (electron microscopy, dynamic light scattering)Knowledge of Pichia pastoris metabolismExperience of working in a multidisciplinary team The following knowledge, skills & experience is also desirable:
Practical experience with cell-free protein synthesis to predict in vivo expression levels of candidate proteinsPractical experience with small-scale fermentation using bioreactors The opportunity to continue your career at a world-leading institution and be part of our mission to continue science for humanity.Grow your career: gain access to Imperial’s sector-leading as well as opportunities for promotion and progression. Sector-leading salary and remuneration package (including 39 days off a year and generous pension schemes). Be part of a diverse, inclusive and collaborative work culture with various and resources to support your personal and professional .
